Rabbit Polyclonal IgG Anti-Collagen Type II, mouse
633 EUR
0.5 ml
CO20251
Please contact us to check the availability
Purified collagen type II from fetal murine cartilage
Murine collagen type II 100%, murine collagen type I, III and IV
Mouse, cross-reactivity with bovine collagen type II in ELISA
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Primary Antibody
Rabbit
IgG
polyclonal
purified, lyophilized
IFA ≥ 1:80, IHC(P) ≥ 1:1000, ELISA ≥ 1:200 (OD ≥ 500)
app. 1 mg/ml
Mouse cartilage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
IHC(P), IFA, ELISA, RIA, IB/WB
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
Store at -20°C
P28481 (CO2A1_MOUSE)
1. Begue-Kirn C., Smith A.J., Ruch J.V., Wozney J.M., Purchio A., Hartmann D.J., Lesot H. (1992) Effects of dentin proteins, transforming growth factor ?1 (TGFß1) and bone morphogenetic protein 2 (BMP2) on the differentiation of odontoblast in vitro. Int. J. 2. Ihanamaki T., Salminen H., Saamanen A.M., Pelliniemi L.J., Hartmann D.J., Sandberg-Lall M., Vuorio E. (2001) Age-dependent changes in the expression of matrix components in the mouse eye. Exp. Eye Res. 72, 423-431. 3. Valcourt U., Gouttenoire J., Aubert-Foucher E., Herbage D., Mallein-Gérin F. (2003) Alternative splicing of type II procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF- beta1. FEBS Letters 545, 115-119.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus,Oryctolagus cuniculus
Polyclonals and antibodies
