Goat antibody to or anti-Rat IgG (H&L) - Affinity purified, FITC conjugated Conjugate

Basic information

  • Name

    Goat antibody to or anti-Rat IgG (H&L) - Affinity purified, FITC conjugated Conjugate

  • Price

    188 EUR

  • Size

    1 vial

  • Catalog number

    GWB-FAA914

More detailed information

Old catalog number

25-381-477575

Storage temperature

store cold

Expiry date

1 y

Category

research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use

Test

A high affinity purification column was use to purify Goat antibody anti- IgG (H&L) - FITC conjugated Conjugate by genways by chromatographic size exclusion.

Description

1 of protein A or G purified by genways will give more specificity than crude serum.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by genways they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This genways Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. genways FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Rattus norvegicus

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

French translation

anticorps