DONKEY ANTI RABBIT IgG (H/L):Biotin (HUMAN/RAT/MOUSE ADSORBED) Antibody
486 EUR
1000ug
MBS235091
Anti-DONKEY ANTI RABBIT IgG (H/L):Biotin (HUMAN/RAT/MOUSE ADSORBED)
DONKEY ANTI RABBIT IgG (H/L):Biotin (HUMAN/RAT/Murine ADSORBED)
DONKEY ANTI Lapin IgG (H/L):Biotin (Homo sapiens/Rattus norvegicus/Mus musculus ADSORBED)
IgG
N/A
Ig kappa-b4 chain C region; Ig kappa-b4 chain C region; N/A; N/A; N/A
IgG
N/A
N/A
Antibodies
Polyclonal
Polyclonal IgG
Polyclonal antibody
Donkey
Rabbit
IgG This item is specific for rabbit immunoglobulin G heavy and light chains, and shows minimal cross-reactivity with human, rat and mouse serum proteins. This antibody may also recognise the light chains of other rabbit immunoglobulins.
N/A
Biotin (Purified IgG conjugated to Biotin - liquid)
0.5 mg/ml
Store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. Storage in frost-free freezers is not recommended. productone should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from the date of dispatch.
ELISA (EIA), Immunohistology - Frozen (IHC)
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Biotinylated
Equus africanus asinus,Mus musculus,Oryctolagus cuniculus,Rattus norvegicus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by MBS Polyclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. MBS Polyclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
IgG
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
anticorps
