Anti-Rat IgG (H+L) (mouse absorbed), Biotin, (Polyclonal) (goat IgG)
365 EUR
1 mililiter
MBS524020
Anti-Anti-Rat IgG (H+L) (mouse absorbed), Biotin, (Polyclonal) (goat IgG)
Anti-Rat IgG (H+L) (Murine absorbed), Biotin, (PAb) (Caprine IgG)
Anti-Rattus norvegicus IgG (H+L) (Mus musculus absorbed), Biotin, (Polyclonal) (Caprinae IgG)
IgG (H+L) (mouse absorbed)
IgG (H+L) (mouse absorbed), Biotin, (Polyclonal) (goat IgG); Biotin Goat Anti-Rat IgG (H+L) Mouse Adsorbed
N/A
N/A
N/A
N/A
Antibodies
Polyclonal
N/A
Polyclonal antibody
N/A
Rat
N/A
N/A
N/A
N/A
Stable at Store productone at +4 degrees Celsius.. For long term storage, aliquot and freeze unused portions productone should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles. Mix thoroughly but gently after thawing and before use. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.
ELISA (EIA)
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
Capra aegagrus hircus,Mus musculus,Rattus norvegicus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
IgG
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Polyclonals and antibodies
