Anti-Human IgG (H+L)-FITC

Basic information

  • Name

    Anti-Human IgG (H+L)-FITC

  • Price

    205 EUR

  • Size

    1000ug

  • Catalog number

    MBS674873

More detailed information

Alternative name1

Anti-Rabbit Anti-Human IgG (H+L)-FITC

Alternative name2

Rabbit Anti-Human IgG (H+L)-FITC

Alternative name3

Lapin Anti-Homo sapiens IgG (H+L)-FITC

Alternative name4

Rabbit Anti-Human IgG (H+L)

Alternative name5

Rabbit Anti-Human IgG(H+L) - FITC; Rabbit Anti-Human IgG (H+L)

Other names

N/A

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Category

Secondary Antibodies

Clonality

Polyclonal

Immunoglobulin isotype

IgG

Clone

Polyclonal antibody

Host organism

Rabbit

Species reactivity

Human

Specificity and cross-reactivity

Reacts with the heavy and light chains of human IgG as demonstrated by ELISA.May also react with the light chains of other human immunoglobulins.

Purification method

Affinity chromatography on human IgG covalently linked to Sepharose-4B.

Form/Appearance

Fluorescein (FITC) Conjugate

Concentration

N/A

Storage and shipping

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at The items should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Tested for:

ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot

Description

productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.

Advisory

In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

MBS Polyclonals supplies antibodies that are for research of human proteins.

Properties

This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Anti-FITC Antibody

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG