Anti-Sheep IgG (H&L) FITC

Basic information

  • Name

    Anti-Sheep IgG (H&L) FITC

  • Price

    293 EUR

  • Size

    100ul

  • Catalog number

    MBS826763

More detailed information

Alternative name1

Anti-Rabbit Anti-Sheep IgG (H&L) FITC

Alternative name2

Rabbit Anti-Ovine IgG (H&L) FITC

Alternative name3

Lapin Anti-Ovinae IgG (H&L) FITC

Alternative name4

IgG (H&L) FITC

Alternative name5

N/A

Other names

N/A

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Category

Secondary Antibodies

Clonality

Polyclonal

Immunoglobulin isotype

N/A

Clone

Polyclonal antibody

Host organism

Rabbit

Species reactivity

Sheep

Specificity and cross-reactivity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

Purification method

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Form/Appearance

Liquid in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, containing 1% BSA, 50% glycerol, 0.05% Sodium Azide

Concentration

N/A

Storage and shipping

Shipped at Store productone at +4 degrees Celsius.. Upon delivery aliquot and store productone should be stored at -20 degrees Celsius. for six months. Avoid freeze/thaw cycles.

Tested for:

Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Description

productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.

Advisory

In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.