Anti-Rat IgG H&L) Antibody Cross Adsorbed) - FITC Conjugated

Basic information

  • Name

    Anti-Rat IgG H&L) Antibody Cross Adsorbed) - FITC Conjugated

  • Price

    424 EUR

  • Size

    one vial

  • Catalog number

    OAIB00702

More detailed information

Recognized antigen

Rat IgG H&L) Cross Adsorbed)

Product type

Antibody

Gene name

Rat IgG H&L) Cross Adsorbed)

Long name

Antibody against Rat IgG H&L) Cross Adsorbed)

Storage

For shorter time periods the Rat IgG H&L) Cross Adsorbed) Antibodies may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds of the Rat IgG H&L) Cross Adsorbed) Antibodies which might cause change or loss of the desired properties.

Tips

Small amounts of the Rat IgG H&L) Cross Adsorbed) antibody may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by aviva they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This aviva Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aviva FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus

French translation

anticorps