Biotin donkey anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL
142 EUR
50 µL
20191-1
Biotin donkey anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL
NonCF Dye Antibodies
15°C to 30°C
n/a
-30°C to -2°C
GHS07 exclamation mark, GHS08 systemic health hazards
Warning, Danger
H315 Causes skin irritation H319 Causes serious eye irritation H335 May cause respiratory irritation H373 Causes damage to organs through prolonged or repeated exposure category 2 ( lungs) H372 Causes damage to organs through prolonged or repeated exposure category 1 (gastrointestinal) (oral)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
Equus africanus asinus,Oryctolagus cuniculus
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by Biotium. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biotium adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
