Goat anti Rabbit IgG (heavy and light chains)

Basic information

  • Name

    Goat anti Rabbit IgG (heavy and light chains)

  • Price

    148 EUR

  • Size

    1 mL

  • Catalog number

    GAR/IgG(H+L)

More detailed information

Category

Secondary Antibodies

Long description

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal rabbit serum; to prepare an immunoadsorbent for the production of Ig-free rabbit serum, plasma or non-Ig preparations.

Antibody come from

Purified normal IgG isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Other description

Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.

Clone

Polyclonal

Antigen-antibody binding interaction

Goat anti Rabbit IgG (heavy and light chains) Antibody

Antibody is raised in

Goat

Antibody's reacts with

Rabbit

Antibody's reacts with these species

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine – Dog + Hamster + Mouse ± Rat - Turkey - Cat + Duck - Horse + Monkey + Sheep - Chicken – Guinea Pig + Human + pigeon - Swine +

Antibody's specificity

Precipitating polyclonal Goat antiSerum to Rabbit IgG, heavy and light chains.

Research interest

Veterinary

Application

Immunoprecipitation

Antibody's suited for

Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:128 when tested against normal rabbit serum in agar block titration.

Storage

The lyophilised antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days

Relevant references

no information yet

Protein number

see ncbi

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Capra aegagrus hircus,Oryctolagus cuniculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG