Sheep anti Horse IgG (heavy and light chains)
148 EUR
1 mL
ShAHo/IgG(H+L)
Secondary Antibodies
The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses IgG1 and IgG2. Reactivity with other subclasses of horse IgG has not been tested. The antiserum reacts with both Fc and Fab portions of polyclonal IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against horse serum usually a single characteristic precipitin line is observed, representing primarily IgG2. If the level of IgG1, also known as IgG(T), in the serum sample approaches the level of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in horse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal horse serum; to prepare an immunoadsorbent for the production of Ig-free horse serum, plasma or non-Ig preparations. It has not been tested for use in nephelometry, ELISA or immunohistology, but this does not exclude such use if proper controls are included.
Purified normal IgG isolated from pooled horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. No foreign proteins added.
Polyclonal
Sheep anti Horse IgG (heavy and light chains) Antibody
Sheep
Equine
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Precipitating polyclonal Sheep antiSerum to Horse IgG, heavy and light chains.
Veterinary
Immunoprecipitation
Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:32 when tested against normal horse serum in agar block titration.
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
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This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
IgG
