Rabbit Monoclonal IgG Anti-CD274 (PD-L1) [ZR3]

Basic information

  • Name

    Rabbit Monoclonal IgG Anti-CD274 (PD-L1) [ZR3]

  • Price

    755 EUR

  • Size

    1 ml

  • Catalog number

    CD274-1.0

More detailed information

Availability

Please contact us to check the availability

Immunogen

KLH-conjugated linear peptide from human PD-L1

Specificity

Human CD274, PD-L1

Species reactivity

Human

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

ZR3

Product is supplied as

liquid concentrate

Recommended concentration for use

1:50-1:100

Protein concentration

N/A

Positive control

Placenta, lung carcinoma (NSCLC)

Pretreatment

Unmasking with Unmasking fluid E (Art. No. DE006) pH 9.0 or Unmasking fluid T (DE005) pH 8.0

Tested applications

IHC(C, P)

Recommended secondary reagent

Anti-Rabbit IgG-conjugates (z.B. VECTOR BA-1000) or VECTOR ImmPress Rabbit IgG (MP-7401)

Storage method

Store at

UniProt number

Q9NZQ7 (PD1L1_HUMAN)

Litterature

1. Ishida, Agata, Shibahara et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. The EMBO journal, Vol. 11, Issue 11, pp. 3887-95 (PubMed). 2. Wang W, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2016) IFN-γ up-regulates PD-L1 expression in human placenta mesenchymal stem cells and enhances cell ability to induce the differentiation of IL-10+ T cells from cord blood- and peripheral blood-derived T cells. PMID 26927379 3. Benjamin J. Chen, Bjoern Chapuy, Jing Ouyang, et al. (2013) PD-L1 Expression Is Characteristic of a Subset of Aggressive B-cell Lymphomas and Virus-Associated Malignancies Clinical Cancer Research. CCR-13-0855

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus