Rabbit Polyclonal IgG Anti-Beta-Defensin-2 (HBD-2/MBD-2)
0 EUR
100 µg
DEF02-A
Please contact us to check the availability
Mixture of synthetic peptides (14 AA near N-terminus of the mature HBD-2, 14 AA near N-terminus of the mature rat ß-Defensin-2, and 17 AA near N-terminus of the mature mouse ß-Defensin-2) coupled to KLH for immunisation of rabbits
ß-defensin-2 (HBD-2) whole molecule and N-terminal fragments of HBD-2, no cross-reactivity to alpha-defensin and other beta-defensins, full cross-reaction with mouse and rat beta-defensin-2
Human, mouse, rat, other species not tested
Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
Primary Antibody
Rabbit
IgG
polyclonal
lyophilisate
1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)
N/A
Skin or oral mucosa
ELISA, RIA, IHC not established
We recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (Ref. PK-6101) or BioPrime Universal Staining Kits (Ref. AE005 and DA005).
Store at -20'°C'
Go to UniProt/SwissProt
1. Harder J., Bartels J., Christophers E., and J.-M. Schröder (1997) Antibacterial peptide specific for Gram-negative Bacteria / also effective for Candida albicans. Nature 387; 861ff 2. Hiratsuka T., Nakazato M., Date Y., Ashitani J., Minematsu T, Chino N., and S. Matsukura (1998) Biochem. Biophys. Res. Comm. (Pharmacol.) 249; 943 ff
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
Polyclonals and antibodies
Oryctolagus cuniculus
