Rabbit Polyclonal IgG Anti-Beta-Defensin-3 (HBD-3/MBD-3), affinity purified
0 EUR
100 µg
DEF03-A
Please contact us to check the availability
Mixture of synthetic peptides 15 aa near C-terminus of the mature HBD-3 (LPKEEQIGKCSTRGR) and 13 aa of N-terminal MBD-3, coupled to KLH for immunisation of rabbits
ß-defensin-3 of human and mouse origin, N-terminal fragments of HBD-3 and MBD-3, no cross-reactivity with other known ß-defensins or alpha-defensins
Human, mouse
Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
Primary Antibody
Rabbit
IgG
polyclonal
lyophilisate
1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)
N/A
Skin and airway epithelia
ELISA, RIA, IHC not established
We recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (Ref. PK-6101) or BioPrime Universal Staining Kits (Ref. AE005 and DA005).
Store at -20'°C'
Go to UniProt/SwissProt
1. Harder J., Bartels J., Christophers E., and J.-M. Schröder (1997) Antibacterial peptide specific for Gram-negative Bacteria / also effective for Candida albicans. Nature 387; 861ff 2. Bals R., Wang X., Meegalla R. L., Wattler S., et al. (1999) Mouse ß-Defensin 3 is an inducible antimicrobial peptide expressed in the epithelia of multiple organs. Infection and Immunity 67(7); 3542-3547. 3. Jia H.P., Schutte B.C., Schudy A., Linzmeier R., et al. (2001) Discovery of new human beta-defensins using a genomics-based approach. Gene 263(1-2);211-218. 4. Garcia J.R., Jaumann F., Schulz S., Krause A., et al. (2001) Identification of a novel multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 306(2); 257-264. 5. Harder J., Bartels J., Christophers E., and J.-M. Schröder (2001) Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic. J. Biol. Chem. 276(8); 5707-5713.
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
A high affinity purification column was use to purify Research by bio logo by chromatographic size exclusion.
1 of protein A or G purified by bio logo will give more specificity than crude serum.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
Polyclonals and antibodies
Oryctolagus cuniculus
