Rabbit Polyclonal IgG Anti-Elastin, human

Basic information

  • Name

    Rabbit Polyclonal IgG Anti-Elastin, human

  • Price

    448 EUR

  • Size

    0.5 ml

  • Catalog number

    EL25011

More detailed information

Availability

Please contact us to check the availability

Immunogen

Purified elastin from human skin

Specificity

Human elastin, cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V

Species reactivity

Human, cross-reaction with rat < 0.1%

Product presentation

Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

polyclonal

Product is supplied as

purified, lyophilized

Recommended concentration for use

IFA ≥ 1:80, IHC(P) ≥ 1:500, ELISA ≥ 1:200 (OD ≥ 500)

Protein concentration

app. 1 mg/ml

Positive control

Human skin or aorta

Pretreatment

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Tested applications

IFA, IHC(P), ELISA, RIA

Recommended secondary reagent

Anti-rabbit IgG-conjugates

Storage method

Store at -20°C

UniProt number

P155Go to UniProt/SwissProt2 (ELN_HUMAN)

Litterature

1. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 2. Guerret S., Govignon E., Hartmann D.J., Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 3. Zsolt U., Peyrol S., Plauchu H., Zabot M.T., Lebwohl M., Schilling K., Green M., Boyd C.D., Csiszár K. (2000) Elastin gene deletions in Williams syndrome patients result in altered deposition of elastic fibers in skin and a subclinical dermal phenotype. P 4. Duplan-Perrat F., Damour O., Montrocher C., Peyrol S., Grenier G., Jacob M.P., Braye F. (2000) Keratinocytes influence the maturation and organization of the elastin network in a skin equivalent. J. Invest Dermatol 114, 365-370.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus