Rabbit Polyclonal IgG Anti-Elastin, rat
575 EUR
0.5 ml
EL25041
Please contact us to check the availability
Purified elastin from rat aorta
Rat elastin 100%, rat collagen type I, II, III and V
Rat, cross-reaction with mouse elastin quite strong (IHC skin, liver) cross-reaction with human elastin (RIA)
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Primary Antibody
Rabbit
IgG
polyclonal
purified, lyophilized
IFA ≥ 1:40, IHC(P) ≥ 1:500, ELISA ≥ 1:200 (OD ≥ 500)
app. 1 mg/ml
Rat skin or aorta
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
IFA, IHC(P), ELISA, RIA
Anti-rabbit IgG-conjugates
Store at -20°C
Q99372 (ELN_RAT)
1. Desmoulière A., Darby I., Monte Alto Costa A., Raccurt M., Tuchweber B., Sommer P., Gabbiani G. (1997) Extracellular Matrix Deposition, lysyl oxidase expression, and myofibroblastic differentiation during the initial stages of cholestatic fibrosis in the rat. Lab Invest.76(6):765-78. 2. Lethias C., Chignier E., Garrone R., Hartmann D.J., Eloy R. (1988) Elastogenesis in rat arterial grafts : elastin deposits on microfibrillar and non-microfibrillar structures. Histochem. J. 20, 715-721. 3. Ruggiero F., Lethias C., Garrone R. (1987) Immunolabelling of freeze-fractured tissue : thin sections of rat aorta after labelling of elestic laminae. Eur. J. Cell. Biol. 44, 90-92. 4. Lethias C., Hartmann D.J., Masmejean M., Ravazzola M., Sabbagh I., Ville G., Herbage D., Eloy R. (1987) Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique. J. Histochem. Cytochem. 35, 15-21.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
IgG
Polyclonals and antibodies
Oryctolagus cuniculus,Rattus norvegicus
