Rabbit Monoclonal IgG Anti-GCDFP-15 (BRST-2, PIP) [EP1582Y]

Basic information

  • Name

    Rabbit Monoclonal IgG Anti-GCDFP-15 (BRST-2, PIP) [EP1582Y]

  • Price

    482 EUR

  • Size

    0,5 ml

  • Catalog number

    GCD002-0.5

More detailed information

Availability

Please contact us to check the availability

Immunogen

Synthetic peptide corresponding to N-Terminus of human GCDFP

Specificity

GCDFP-15 (gross cystic disease fluid protein 15), BRST-2, PIP (prolactin-inducible protein)

Species reactivity

Human, others not known

Product presentation

Purified antibody in PBS, BSA, Na-Azid 0,09%

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

EP1582Y

Product is supplied as

concentrate

Recommended concentration for use

1:50-1:100

Protein concentration

N/A

Positive control

Breast, skin, salivary gland

Pretreatment

Pretreatment of deparaffinized tissue with heat induced epitope retrieval is recommended (Unmasking fluids: DE000, DE007, DE005).

Tested applications

IHC (C, P)

Recommended secondary reagent

Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art.-No. FI-1000) or anti-rabbit IgG:DyLight488 (Art.-No. DI-1488). Best results for paraffin slides with polymer systems like DAKO Envision or VECTOR ImmPress ™ (Art.-No. MP-7401).

Storage method

Store at 2-8°C

UniProt number

P12273 (PIP_HUMAN)

Litterature

1. Murphy L.C., Tsuyuki D., Myal Y., Shiu R.P.C. (1987) Isolation and sequencing of a cDNA clone for a prolactin-inducible protein (PIP). Regulation of PIP gene expression in the human breast cancer cell line, T-47D. J. Biol. Chem. 262:15236-15241(1987) 2. Naderi A (2015) Prolactin-induced protein in breast cancer. Adv Exp Med Biol. 2015;846:189-200. 3. Armand Asarian, Olubunmi Esan, Philip Xiao, Segun Adeoye (2014) Cutaneous metastatic breast adenocarcinoma twenty years post-mastectomy: A lesson to learn International Journal of Case Reports and Images, Vol. 5 No. 9, September 2014. ISSN – [0976-3198 4. Darb-Esfahani S1, von Minckwitz G, Denkert C, Ataseven B, Högel B, Mehta K, Kaltenecker G, Rüdiger T, Pfitzner B, Kittel K, Fiedler B, Baumann K, Moll R, Dietel M, Eidtmann H, Thomssen C, Loibl S. (2014) Gross cystic disease fluid protein 15 (GCDFP-15) 5. Rohit Bhargava, MD1; Sushil Beriwal, MD2; David J. Dabbs, MD1 (2007) Mammaglobin vs GCDFP-15: An Immunohistologic Validation Survey for Sensitivity and Specificity Am J Clin Pathol. 2007;127(1):103-113. 

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus