Sheep Polyclonal IgG Anti-IgG4, human, FITC-conjugated
703 EUR
0.5 ml
IGG4001-F
Please contact us to check the availability
Purified human IgG4
human IgG4
Human
Affinity purified antibody conjugated to FITC in PBS, pH 7.2, 1% BSA, 0.099% sodium azide
Primary Antibody
Sheep
IgG
polyclonal
liquid concentrate
1:20 - 1:100
0.5 mg/ml
IEP, WB, FIA, Immunohistochemistry, Fluorescent Microscopy
Store at 2-8°C
1. Kleger A, Seufferlein T, Wagner M, Tannapfel A, Hoffmann TK, Mayerle J (2015) IgG4-associated autoimmune diseases—polymorphous presentation complicates diagnosis and treatment. Dtsch Arztebl Int; 112: 128–35. 2. Stone et al. (2012). Recommendations for the nomenclature of IgG4-related disease and its individual organ system manifestations. Arthritis & Rheumatism. 64 (10): 3061–3067. 3. Deshpande et al. (2012). Consensus statement on the pathology of IgG4-related disease. Modern Pathology. 25 (9): 1181–1192. 4. A. Fernandez-Flores (2012) The role of IgG4 in cutaneous pathology. Rom J Morphol Embryol, 53(2):221-231.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
This bio logo Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. bio logo FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Anti-FITC Antibody
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
IgG
Polyclonals and antibodies
