Rabbit/Mouse Monoclonal IgG Anti-PIN-Cocktail 1B -- P504S / p63 [13H4/EPR5701]

Basic information

  • Name

    Rabbit/Mouse Monoclonal IgG Anti-PIN-Cocktail 1B -- P504S / p63 [13H4/EPR5701]

  • Price

    946 EUR

  • Size

    1 ml

  • Catalog number

    PIN103-1.0

More detailed information

Availability

Please contact us to check the availability

Immunogen

1. Recombinant human AMACR (P504S) protein, whole sequence; 2. Recombinant human p63 protein aa 1-203

Specificity

Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase and human p63 nuclear protein

Species reactivity

Human

Product presentation

Affinity purified antibodies in PBS, BSA, sodium azide (0.09%)**. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.

Product category

Primary Antibody

Antibody's raised in

Rabbit/Mouse

Antibody's isotype

IgG

Antibody's clone

13H4/EPR5701

Product is supplied as

liquid concentrate

Recommended concentration for use

1:25-1:50

Protein concentration

N/A

Positive control

Prostatic intraepithelial neoplasia

Pretreatment

Use formalin-fixed and paraffin-embedded sections; Retrieval conditions: Unmasking fluid T, TEC buffer (Tris/EDTA/Citrate) pH 8 (Art. No. DE005) in a pressure cooker at 100°C 20-40 minutes

Tested applications

IHC(C, P)

Recommended secondary reagent

Detection methods: VECTASTAIN Universal Elite ABC or ImmPRESS Rabbit IgG or other polymer kit detecting Rabbit IgG.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Jiang Z, Woda BA, Rock KL et al. (2001) P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol 25:1397-1404. 2. Weinstein MH, Signoretti S, Loda M (2002) Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod Pathol. Dec;15(12):1302-8. 3. Luo J, Shan Zha, Wesley R, et al. (2002) Alpha methylacyl-CoA recemase, a new molecular marker for prostate cancer. Cancer Res 62:2220-2226.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus,Oryctolagus cuniculus