Rabbit Polyclonal IgG Anti-Survivin (IAP)

Basic information

  • Name

    Rabbit Polyclonal IgG Anti-Survivin (IAP)

  • Price

    262 EUR

  • Size

    5 ml

  • Catalog number

    SUR002-G

More detailed information

Availability

Please contact us to check the availability

Immunogen

Synthetic C-terminal peptide sequence of human Survivin

Specificity

Human Survivin

Species reactivity

Human, mouse, others not tested

Product presentation

Affinity purified IgG from rabbit serum in PBS, BSA, NaN3

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

polyclonal

Product is supplied as

ready to use

Recommended concentration for use

neat

Protein concentration

N/A

Positive control

Pancreas (alpha-cells), Prostate carcinoma

Pretreatment

After formaldehyde fixation antigen unmasking at 96-100°C is recommended (use citrate buffer Art. No. DE000 or Unmasking Fluid G on glycol basis Art. No. DE007 respectively).

Tested applications

IHC(P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), for higher sensitivities VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

O15392

Litterature

1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 1997 Aug;3(8):917-21. 2. Liggins C et al. (2003) Pediatric Dev. Pathol. 6: 392-397. 3. Kuar P., et al. (2004) Arch. Pathol. Lab. Med. 128: 39-43.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus