Rabbit Polyclonal IgG Anti-VEGF-A, murine

Basic information

  • Name

    Rabbit Polyclonal IgG Anti-VEGF-A, murine

  • Price

    320 EUR

  • Size

    100 µg

  • Catalog number

    VEGA03

More detailed information

Availability

Please contact us to check the availability

Immunogen

Highly purified recombinant murine VEGF164

Specificity

VEGF-A isoforms in mouse and humans (Western Blot under reducing and non-reducing conditions)

Species reactivity

Human, Mouse

Product presentation

Protein A purified IgG lyophilised from PBS pH 7.4 without preservative

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

polyclonal

Product is supplied as

lyophilisate

Recommended concentration for use

Direct ELISA 0.5-5 µg/ml, Western Blot 0.1-0.2 µg/ml, IHC 0.5-2 µg/ml

Protein concentration

100 µg

Positive control

Metastasing tumours of different origin

Pretreatment

Formaldehyde fixed tissue shows good results after pre-treatment with proteinase K (0.01%, 15 min at RT), other proteases and micro-wave techniques are not validated.

Tested applications

IHC(C, P), ELISA, WB

Recommended secondary reagent

As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)

Storage method

Store at -20°C

UniProt number

QGo to UniProt/SwissProtGo to UniProt/SwissProt731 (VEGFA_MOUSE)

Tips

Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus