Rabbit Polyclonal IgG Anti-VEGF-A, murine
355 EUR
50 µg
VEGA03A
Please contact us to check the availability
Highly purified recombinant murine VEGF164
VEGF-A isoforms in mouse and humans (Western Blot under reducing and non-reducing conditions)
Human, Mouse
Peptide affinity purified using murine VEGF164 as matrix. Lyophilised from PBS pH 7.4 without preservative.
Primary Antibody
Rabbit
IgG
polyclonal
lyophilisate
Direct ELISA 0.5-5 µg/ml, Western Blot 0.1-0.2 µg/ml, IHC 0.5-2 µg/ml
50 µg
Metastasing tumours of different origin
Formaldehyde fixed tissue shows good results after pre-treatment with proteinase K (0.01%, 15 min at RT), other proteases and micro-wave techniques are not validated.
IHC(C, P), ELISA, WB
As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)
Store at -20°C
QGo to UniProt/SwissProtGo to UniProt/SwissProt731 (VEGFA_MOUSE)
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
Polyclonals and antibodies
Oryctolagus cuniculus
