Chicken anti Goat IgG H + L biotin

Basic information

  • Name

    Chicken anti Goat IgG H + L biotin

  • Price

    327 EUR

  • Size

    1 mg

  • Catalog number

    43R-1034

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Goat IgG (H&L)

Raised in

Chicken

Specificity

This antibody reacts with heavy chains on Goat IgG and light chains on all Goat immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin Goat serum proteins.

Conjugate/Tag

Biotin

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Goat IgG (H&L)

Source

Ig fraction was prepared using eggs obtained from healthy hens of US origin.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG