Chicken anti Rabbit IgG H + L biotin

Basic information

  • Name

    Chicken anti Rabbit IgG H + L biotin

  • Price

    241 EUR

  • Size

    500 µg

  • Catalog number

    43R-1434

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Rabbit IgG (H&L)

Raised in

Chicken

Specificity

This antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin rabbit serum proteins or IgG from human or mouse.

Conjugate/Tag

Biotin

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Rabbit IgG (H&L)

Source

Ig fraction was prepared using eggs obtained from healthy hens of US origin.

Concentration

0.5 mg/ml (lot specific)

Form & Buffer

Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.

Conjugation

Biotinylated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus