Donkey anti Rabbit IgG H + L FITC

Basic information

  • Name

    Donkey anti Rabbit IgG H + L FITC

  • Price

    327 EUR

  • Size

    2 mg

  • Catalog number

    43R-1443

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Rabbit IgG (H&L)

Raised in

Donkey

Specificity

This antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or serum proteins.

Conjugate/Tag

FITC

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Rabbit IgG (H&L)

Source

Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

FC; IF; IHC

Usage Recommendations

The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Latin name

Equus africanus asinus,Oryctolagus cuniculus

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG