Mouse anti Rat IgG Fc HRP

Basic information

  • Name

    Mouse anti Rat IgG Fc HRP

  • Price

    290 EUR

  • Size

    100 µl

  • Catalog number

    43R-1594

More detailed information

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Rat IgG antibodies were raised in mouse using Rat IgG Fc region ..

Raised in

Mouse

Specificity

Rat Fc

Clone

Mixture

Conjugate/Tag

HRP

Method of Purification

Rat IgG antibody was purified by Protein A chromatography.

Form & Buffer

Provided in PBS containing 0.01% thimerosal as a preservative

Storage

Store at 4 deg C for up to 1 month. For long term storage, aliquot and store at -20 to -80 deg C. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; WB

Usage Recommendations

ELISA: 1:2000; WB: 1:1000-1:10000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Conjugation

HRP Conjugated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus,Rattus norvegicus