Rabbit anti Rat IgG H + L Fab'2

Basic information

  • Name

    Rabbit anti Rat IgG H + L Fab'2

  • Price

    389 EUR

  • Size

    500 µg

  • Catalog number

    41C-CJ0307

More detailed information

Category

Secondary Antibody

Antibody Subtype

Purified Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Rabbit anti-rat IgG (H+L) (Fab'2) was raised in rabbit using rat IgG whole molecule ..

Raised in

Rabbit

Specificity

Rat IgG (H + L)

Cross Reactivity

Should be determined by the end user

Method of Purification

Rabbit anti Rat IgG (H + L) (Fab'2) was purified by affinity chromatography.

Concentration

1.0 mg/ml

Form & Buffer

0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.

Storage

Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC

Usage Recommendations

ELISA: 1:60,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent

Additional isotype

 Fab fragment,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Oryctolagus cuniculus,Rattus norvegicus