FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*

Basic information

  • Name

    FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*

  • Price

    345 EUR

  • Size

    1 mg

  • Catalog number

    16860

More detailed information

Description

FITC goat anti-mouse IgG (H+L) *Cross Adsorbed* is an orange solid soluble in water. It is an antibody that reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. It can provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.

Shipping

Blue Ice

Storage

Freezer

Shelflife

12 months

Molecular Weight

NA

Appearance

Orange

Solubility

Soluble in: Water

Excitation Wavelength

492 nm

Emission Wavelength

515 nm

Personal precautions

Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.

Uses

Laboratory chemicals *For Research Use Only*

Ordering

To order FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*, please use the Cat. Nr.16860 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information

Tips

Our specialists recommend you to follow carefully the pre-registered instructions for FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Mus musculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.