Alkaline Phosphatase Conjugated anti-Mouse/Rabbit IgG SABC Kit
300 EUR
1 kit
SA1050
NA
4°C for one year. Avoid freezing
1. 5% BSA Blocking Reagent: 12 ml, for blocking tissue sections. 2. Biotinylated Secondary Antibody (Goat Anti-mouse/anti-rabbit IgG): 12ml (10ug/ml). Affinity purified antibody, labeled with “long-arm†biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). 3. SABC-AP (Alkaline Phosphatase conjugated streptavidin): 12ml (20ug/ml). Manufactured by Boster’s proprietary method, the complex is very stable and offers superior amplification of the antigen signals. 4. BCIP/NBT Chromogenic agent (20x): 1ml. 5. Water soluble sealing reagent: 12 ml. 6. Neutral nuclear fast red: 12 ml.
IHC, ICC
NA
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by boster. Rabbit antibodies are very stable and can be stored for several days at room temperature. boster adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus,Oryctolagus cuniculus
