Rabbit Anti-Rat IgG Secondary Antibody, Biotin Conjugate

Basic information

  • Name

    Rabbit Anti-Rat IgG Secondary Antibody, Biotin Conjugate

  • Price

    240 EUR

  • Size

    2X0.5ml

  • Catalog number

    BA1005

More detailed information

Clonality

Polyclonal

Concentration

1mg/ml

Conjugate

Biotin

Immunogen

Rat IgG (whole molecule).

Storage & Transport Conditions

At 4°C for one year.

Applications

ELISA, IHC, ICC, WB

Reactivity

Rat

Product Datasheet

www.bosterbio.com/datasheet.php?sku=BA1005

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by boster. Rabbit antibodies are very stable and can be stored for several days at room temperature. boster adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus,Rattus norvegicus

French translation

anticorps