FITC Donkey Anti Rabbit IgG (H+L) Secondary Antibody
104 EUR
100ul
AS042
Secondary Antibody
IgG
Secondary Antibody
none
FITC
Immunofluorescence, Flow cytometry
To order the FITC Donkey Anti Rabbit IgG (H+L) Secondary Antibody (Cat. ID#AS042), please submit a purchase order by fax or e-mail. Please contact us for bulk or larger quantity.
Please follow carefully the instructions before every use. Check the vial for the expiry date and batch number. If you need technical assistance please do not hesitate to contact us.
For Research Use Only, not for therapeutic or diagnostic purposes. Not for human or animal consumption.
Please contact our technical support to request the latest datasheet, protocol, certificate of analysis, MSDS.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by ABclonal they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This ABclonal Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. ABclonal FITC is highly sensitive to pH extremes.
Equus africanus asinus,Oryctolagus cuniculus
Anti-FITC Antibody
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by ABclonal. Rabbit antibodies are very stable and can be stored for several days at room temperature. ABclonal adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
IgG
anticorps
