Chicken anti-Rat IgG (H&L) - Affinity Pure, Biotin Conjugate, min x w/human or rabbit IgG or serum proteins[IgG (H&L)]

Basic information

  • Name

    Chicken anti-Rat IgG (H&L) - Affinity Pure, Biotin Conjugate, min x w/human or rabbit IgG or serum proteins[IgG (H&L)]

  • Price

    5 EUR

  • Size

    NA

  • Catalog number

    MBS687668

More detailed information

Products_type

Secondary Antibody

Reactivity

Rat.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin rat serum proteinsserum proteins from human or rabbitIgG from human or rabbit

Tissue

serum

Test

A high affinity purification column was use to purify anti- IgG (H&L) - Pure, Conjugate, min x w/ rabbit IgG serum proteins[IgG (H&L)] by MyBioSource by chromatographic size exclusion.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure anti- IgG (H&L) - Pure, Conjugate, min x w/ rabbit IgG serum proteins[IgG (H&L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Biotinylated

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.Serum should be stored at +5°C

Latin name

Oryctolagus cuniculus,Rattus norvegicus

Group

Sera