Rabbit Anti-IgG Fab fragment Antibody (Biotin)

Basic information

  • Name

    Rabbit Anti-IgG Fab fragment Antibody (Biotin)

  • Price

    423 EUR

  • Size

    100 μg

  • Catalog number

    abx106151

More detailed information

Category

Secondary Antibodies

Clonality

Polyclonal

Raised in

Rabbit

Target

IgG Fab fragment Antibody

Immunogen

see included datasheet or contact us

Species reactivity

Human, Mouse, Rat (for other species please inquire)

Purification

Affinity purified

Applications

WB (for other applications please contact us)

Stock availability

Shipped within 5-10 working days.

Storage conditions

Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.

Footnote

This product is for research use only.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by abbex they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent

Conjugation

Biotinylated

Additional isotype

 Fab fragment,Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by abbex. Rabbit antibodies are very stable and can be stored for several days at room temperature. abbex adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

French translation

anticorps