Mouse Anti-Human HSP70/HSC70 Monoclonal IgG, Clone N27F3-4
464 EUR
200 µg
SMC-104B-A655
N27F3-4
Recombinant HSP70/HSC70
Mouse Anti-Human HSP70/HSC70 Monoclonal IgG Antibody, Clone: N27F3-4: ATTO 655
Monoclonal Antibodies
HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Mouse
HSP70/HSC70
Cancer, Heat Shock
None
WB, IHC, ICC/IF, IP, FCM, IEM
Human, Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Beluga, Dog, Chicken, Fruit Fly (Drosophila melanogaster), Fish, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Plant, Cucumber, Pea (Pisum sativum), Rabbit, Sheep, African clawed frog (Xenopus laevis)
WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Protein G Purified
PBS pH7.2, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~72 (HSP) and ~73kDa (HSC).
-20ºC
Blue Ice or 4ºC
1 µg/ml of SMC-104 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cytoplasm
See included datasheet or contact our support service
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at .
1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993), J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990), Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Polanonka-Grabowska R. et. al. (1997) Blood 90: 1516-1526. 8. Schnell D.J. et. al. (1994) Science 266: 1007-1012. 9. Kabakov A.E., et. al. (2002) Am. J.Physiol. 283(2): C521-C534. 10. Ricart J. et. al. (1997) Biochem. J. 324: 635-643. 11. Hang H. and Fox M.H. (1995) Cytometry 19(2): 119-125.
1-Jan-2007
24447452, 24447452, 23958398, 22831866, 22504172, 22194796, 21445704, 20435172
Western Blot, Immunohistochemistry, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot
Human, Human, Rat, Human, Human, Rat, Hamster, Human
NP_005336.3
3303
P08107
Contact our support service
Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 (SMC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 (SMC-104). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-104) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp70 Antibody [N27] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-104) Mouse Anti-Hsp70 Antibody [N27] used in Western Blot (WB) on Human Cell lysates (SMC-104)
Non-hazardous
Canada
1.4
0.2
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
IgG
Mus musculus
