Mouse Anti-Human HSP70/HSC70 Monoclonal IgG, Clone N27F3-4

Basic information

  • Name

    Mouse Anti-Human HSP70/HSC70 Monoclonal IgG, Clone N27F3-4

  • Price

    448 EUR

  • Size

    200 µg

  • Catalog number

    SMC-104B-FITC

More detailed information

Clone

N27F3-4

Immunogen

Recombinant HSP70/HSC70

Antibody's full description

Mouse Anti-Human HSP70/HSC70 Monoclonal IgG Antibody, Clone: N27F3-4: FITC

Antibody's category

Monoclonal Antibodies

Antibody's other name

HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody

Raised in

Mouse

Antibody's target

HSP70/HSC70

Primary research fields

Cancer, Heat Shock

Brandname

None

Antibodies' applications

WB, IHC, ICC/IF, IP, FCM, IEM

Antibody's reactivity

Human, Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Beluga, Dog, Chicken, Fruit Fly (Drosophila melanogaster), Fish, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Plant, Cucumber, Pea (Pisum sativum), Rabbit, Sheep, African clawed frog (Xenopus laevis)

Antibody's dilutions

WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Purity

Protein G Purified

Antibody buffer for storage

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~72 (HSP) and ~73kDa (HSC).

Storage recommendations

-20ºC

Shipping recommendations

Blue Ice or 4ºC

Antibody certificate of analysis

1 µg/ml of SMC-104 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm

Tissue specificity

See included datasheet or contact our support service

Scientific context

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at .

Bibliography

1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993), J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990), Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Polanonka-Grabowska R. et. al. (1997) Blood 90: 1516-1526. 8. Schnell D.J. et. al. (1994) Science 266: 1007-1012. 9. Kabakov A.E., et. al. (2002) Am. J.Physiol. 283(2): C521-C534. 10. Ricart J. et. al. (1997) Biochem. J. 324: 635-643. 11. Hang H. and Fox M.H. (1995) Cytometry 19(2): 119-125.

Released date

1-Jan-2007

PubMed number

24447452, 24447452, 23958398, 22831866, 22504172, 22194796, 21445704, 20435172

Tested applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot

Tested reactivity

Human, Human, Rat, Human, Human, Rat, Hamster, Human

NCBI number

NP_005336.3

Gene number

3303

Protein number

P08107

Antibody's datasheet

Contact our support service

Representative figure link

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Representative figure legend

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 (SMC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 (SMC-104). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-104) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp70 Antibody [N27] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-104) Mouse Anti-Hsp70 Antibody [N27] used in Western Blot (WB) on Human Cell lysates (SMC-104)

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.2

Stock availability

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Additional isotype

IgG

Latin name

Mus musculus