anti-Goat Anti-Rat IgG (H&L) Biotin

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Basic information

  • Name

    anti-Goat Anti-Rat IgG (H&L) Biotin

  • Price

    295 EUR

  • Size

    Goat Anti-Rat IgG (H&L) Biotin

  • Catalog number

    6910-250

  • Buy online

More detailed information

Target of this antibody:

Rat IgG

Alternative names of antibody target:

Anti-Rat IgG, Biotin-Linked Antibody, Biotin linked secondary antibody

Background information:

Goat Polyclonal Secondary Antibody to Rat IgG (H&L) Biotin.

Antibody host:

Goat

Clonality of antibody:

Secondary antibody

Isotype:

Rat IgG

Clone:

N/A

This antibody reacts with:

Rat

Immunogene sequence:

Rat IgG (H&L)

Concentration of antibody:

N/A

Form of antibody:

Liquid

Formulation of this antibody:

In 0.01 M Sodium phosphate, 0.25 M NaCl, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.

Purification method:

Affinity Purified

This antibody has been succesfully tested for use in following methods:

IF, FACS

Recommended usage:

ELISA: 1:20,000 – 1:400,000, WB: 1:20,000 – 1:400,000, IH: 1:500 – 1:5000, IC: 1:500 – 1:5000.

Storage information:

Shelf time: 6 months in temp.: -20 deg.C

Shipping conditions:

gel pack

How to handle this antibody:

The antibody solution should be gently mixed before use.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Rattus norvegicus

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.