anti-Goat Anti-Human IgG (H&L) FITC

Basic information

  • Name

    anti-Goat Anti-Human IgG (H&L) FITC

  • Price

    349 EUR

  • Size

    Goat Anti-Human IgG (H&L) FITC

  • Catalog number

    6915-100

More detailed information

Target of this antibody:

Human IgG

Alternative names of antibody target:

Anti-human IgG, FITC-Linked Antibody, FITC linked secondary antibody

Background information:

Goat Polyclonal Secondary Antibody to Human IgG (H&L) FITC.

Antibody host:

Goat

Clonality of antibody:

Secondary antibody

Isotype:

Human IgG

Clone:

N/A

This antibody reacts with:

Human

Immunogene sequence:

Human IgG (H&L)

Concentration of antibody:

N/A

Form of antibody:

Liquid

Formulation of this antibody:

In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.

Purification method:

Affinity Purified

This antibody has been succesfully tested for use in following methods:

IF, FACS

Recommended usage:

IF: 1:50 – 1:200, FC: 1:50 – 1:200.

Storage information:

Shelf time: 6 months in temp.: -20 deg.C

Shipping conditions:

gel pack

How to handle this antibody:

The antibody solution should be gently mixed before use.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

Biovision supplies antibodies that are for research of human proteins.

Properties

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.