Anti-Mouse CD3z, Biotin (Clone H146-968) (hamster IgG)
464 EUR
0.3 miligrams
GEN520100
CD3z
CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3; CD247 molecule; T-cell receptor T3 zeta chain
Antibodies
Mnoclonal antibodies
CD3z
N/A
CD247; CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA; CD3Z; T3Z; TCRZ
Monoclonal
Hamster IgG2kappa
H146-968
Hamster
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
CD3z (CD247) (Mouse CD3z (zeta chain); also known as CD247); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
N/A
Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
Antibody Concentration: 0.1 miligram per 1 mililiter
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze the antibody at -20 degrees Celsius. Keep the unused portions of the antibody in volumes suitable for single use. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
Immunohistochemistry (IHC), Immunofluorescence (IF)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
Biotinylated
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
IgG
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus
