MOUSE Anti-HUMAN IgG (Fc) CH2 DOMAIN:FITC Antibody

Basic information

  • Name

    MOUSE Anti-HUMAN IgG (Fc) CH2 DOMAIN:FITC Antibody

  • Price

    486 EUR

  • Size

    100ug

  • Catalog number

    GEN224448

More detailed information

Also known as

IgG

Other names

Ig gamma-3 chain C region; Ig gamma-3 chain C region; N/A; immunoglobulin heavy constant gamma 3 (G3m marker); HDC; Heavy chain disease protein

Category

Antibodies

Subcategory

Mnoclonal antibodies

Gene name

IgG

Gene name synonims

N/A

Other gene names

IGHG3; IGHG3; IgG3; N/A

Clonality

Monoclonal

Immunoglobulin isotype

IgG1

Clone

MK 1 A6

Host organism

Mouse (Mus musculus)

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Specificity and cross-reactivity

IgG This item recognises human IgG Fc (all subclasses). _x000D_Epitope within CH2 domain (determined by haemagglutination and Western blotting using IgG heavy chain and myelomas with defined domain deletions).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Purification method

N/A

Form/Appearance

FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)

Concentration

IgG concentration 0.1 miligram per 1 mililiter

Storage and shipping

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

Tested applications:

Flow Cytometry (FACS)

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test

MBS Monoclonals supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Conjugation

Anti-FITC Antibody

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Latin name

Mus musculus

French translation

anticorps