Anti-Mouse CD3e, FITC (Clone 145-2C11) (hamster IgG)

Basic information

  • Name

    Anti-Mouse CD3e, FITC (Clone 145-2C11) (hamster IgG)

  • Price

    277 EUR

  • Size

    100ug

  • Catalog number

    GEN520097

More detailed information

Also known as

CD3e

Other names

CD3e molecule, epsilon (CD3-TCR complex); N/A

Category

Antibodies

Subcategory

Mnoclonal antibodies

Gene name

CD3e

Gene name synonims

N/A

Other gene names

N/A

Clonality

Monoclonal

Immunoglobulin isotype

Hamster IgG

Clone

145-2C11

Host organism

Hamster

Species reactivity

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Specificity and cross-reactivity

CD3e (Mouse CD3epsilon); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Purification method

N/A

Form/Appearance

FITC

Concentration

Antibody Concentration: 0.1 miligram per 1 mililiter

Storage and shipping

Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze the antibody at -20 degrees Celsius. Keep the unused portions of the antibody in volumes suitable for single use. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity. Avoid prolonged exposure to light.

Tested applications:

Immunohistochemistry (IHC)

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.

Conjugation

Anti-FITC Antibody

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus