Rabbit anti-human-MMP-14 (MT1-MMP) (IgG), polyclonal

Basic information

  • Name

    Rabbit anti-human-MMP-14 (MT1-MMP) (IgG), polyclonal

  • Price

    306 EUR

  • Size

    100 µg/ 100 µl

  • Catalog number

    31 101 004

More detailed information

Antibody category

Polyclonal Antibody

Antibody's description

Rabbit polyclonal antibody against human MMP 14. The polyclonal antibody was raised against a peptide derived from the hinge region between catalytic domain and hemopexin domain of MT1-MMP. For immunization a peptide-KLH-conjugate was used. and IgG fraction were purified by affinity chromatography on a peptide-BSA-matrix.

Raised in

Rabbit

Research area of interest

Diseases associated with MMP14 include Winchester Syndrome and Multicentric Osteolysis, Nodulosis, And Arthropathy. Among its related pathways are Signaling events mediated by focal adhesion kinase and Adhesion

Package form

liquid

Clonality

Polyclonal

Antigen

Peptide derived from the hinge region between catalytic domain and hemopexin domain of MT1-MMP.

Reacts with

Human

Immunogen

See the data sheet or contact us

Contents

20 mM Na-phosphate, pH 7.5, 150 mM NaCl containing 0.01 % thimerosal

Purity

>95%

Tested applications

• ELISA • Western-Blotting with ECL-Detection, suggested dilution 1 : 2000 to detect 100 ng MMP14

Other names

Rabbit polyclonal antibody against human MMP 14. The polyclonal antibody was raised against a peptide derived from the hinge region between catalytic domain and hemopexin domain of MT1-MMP. For immunization a peptide-KLH-conjugate was used. and IgG fraction were purified by affinity chromatography on a peptide-BSA-matrix.

Crossreactivity

no crossreactivty

Antibody specificity

The antibody is specific for MT1-MMP. It does not react to a significant extent with MT2-MMP.

Purification

peptide-BSA-matrix

PubMed citations

See the data sheet

UniProt number

P50281

Storage conditions

- 70°C

Technical datasheet

Contact us to receive the datasheet

Shipping condition

dry ice

Warnings

For Research Use only.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

biotez supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus