Rabbit anti-human-ADAMTS5 (IgG), polyclonal

Basic information

  • Name

    Rabbit anti-human-ADAMTS5 (IgG), polyclonal

  • Price

    377 EUR

  • Size

    100 µg/ 100 µl

  • Catalog number

    31 405 004

More detailed information

Antibody category

Polyclonal Antibody

Antibody's description

Protein G-purified rabbit anti-human ADAMTS5 polyclonal. The IgG fraction was purified by affinity chromatography. The polyclonal antibody was raised against an ADAMTS5-peptide derived from the hinge region between ADAMTS5 disintegrin- and thrombospondin-like domains. For immunization a peptide-KLH-conjugate was used.

Raised in

Rabbit

Research area of interest

Diseases associated with ADAMTS5 include Osteoarthritis and Bone Inflammation Disease. Among its related pathways are Diseases associated with O-glycosylation of proteins and Transport to the Golgi and subsequent modification

Package form

liquid

Clonality

Polyclonal

Antigen

human hinge region between ADAMTS5 disintegrin- and thrombospondin-like domains

Reacts with

Human

Immunogen

See the data sheet or contact us

Contents

20 mM Na-phosphate, pH 7.5, 150 mM NaCl

Purity

>95%

Tested applications

• Western-Blotting with ECL-Detection An antibody dilution of 1/1000 is recommended to detect 5 ng of ADAMTS. • ELISA Suggested dilution range: 1:2000 – 1:4000

Other names

Protein G-purified rabbit anti-human ADAMTS5 polyclonal. The IgG fraction was purified by affinity chromatography. The polyclonal antibody was raised against an ADAMTS5-peptide derived from the hinge region between ADAMTS5 disintegrin- and thrombospondin-like domains. For immunization a peptide-KLH-conjugate was used.

Crossreactivity

low cross reactivity with ADAMTS4

Antibody specificity

reacts primarily with ADAMTS5. When ADAMTS5 and ADAMTS4 are analyzed with the antibody in Western blots and ELISA, ADAMTS5 is detected at 50-fold lower concentrations than ADAMTS4.

Purification

Protein G purified

PubMed citations

See the data sheet

UniProt number

Q9UNA0

Storage conditions

- 70°C

Technical datasheet

Contact us to receive the datasheet

Shipping condition

dry ice

Warnings

For Research Use only.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

biotez supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus