Rabbit anti-Mouse Collagen Type I BrCN Fragment (IgG), polyclonal
246 EUR
100 µg/ 100 µl
31 410 114
Polyclonal Antibody
The antibody was raised against collagen type I BrCN fragment from fetal mouse skin (FMS). The IgG fraction was purified from rabbit neat serum by affinity chromatography.
Rabbit
Diseases associated with collagen I: osteogenesis imperfecta, Ehlers-Danlos Syndrome, Infantile cortical hyperostosis aka Caffey's disease
liquid
Polyclonal
collagen type I BrCN fragment from fetal mouse skin (FMS)
rat, mouse
See the data sheet or contact us
PBS
>95%
• EIA, ELISA, • Flow Cytometry • Immunoblotting, suggested dilution 2 µg/ml-8 µg/ml • Immunofluorescence • Immunohistochemistry • Immunoprecipitation
The antibody was raised against collagen type I BrCN fragment from fetal mouse skin (FMS). The IgG fraction was purified from rabbit neat serum by affinity chromatography.
weak with bovine and very moderately reactivity with human and weak with bovine collagen type IX. No cross reactivity with collagen type II.
reacts with collagen type I of rat and mouse origin. It exhibits weak with bovine and very moderately reactivity with human and weak with bovine collagen type IX. No cross reactivity with collagen type II
Protein A purified
See the data sheet
N/A
- 70°C
Contact us to receive the datasheet
dry ice
For Research Use only.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.IgG
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus,Oryctolagus cuniculus
Polyclonals and antibodies
