Rabbit anti-rat-Collagen Type IX (IgG), polyclonal

Basic information

  • Name

    Rabbit anti-rat-Collagen Type IX (IgG), polyclonal

  • Price

    246 EUR

  • Size

    100 µg/ 100 µl

  • Catalog number

    31 410 804

More detailed information

Antibody category

Polyclonal Antibody

Antibody's description

The polyclonal antibody was raised against collagen type IX from rat chondrosarcoma. It was purified from rabbit neat serum by affinity chromatography.

Raised in

Rabbit

Research area of interest

Diseases associated with collagen IX: EDM2 and EDM3

Package form

liquid

Clonality

Polyclonal

Antigen

collagen type IX from rat chondrosarcoma

Reacts with

rat, mouse, bovine, human

Immunogen

See the data sheet or contact us

Contents

PBS

Purity

>95%

Tested applications

• EIA • ELISA, • Flow Cytometry • Immunoblotting, suggested dilution 300 ng/ml - 1200 ng/ml • Immunofluorescence • Immunohistochemistry, suggest dilution 5 µg/ml - 15 µg/ml • Immunoprecipitation, suggest dilution 10 µg/ml - 30 µg/ml

Other names

The polyclonal antibody was raised against collagen type IX from rat chondrosarcoma. It was purified from rabbit neat serum by affinity chromatography.

Crossreactivity

no crossreactivty

Antibody specificity

reacts with collagen type IX from rat, mouse, human and bovine. It exhibits no cross reactivity

Purification

Protein A purified

PubMed citations

See the data sheet

UniProt number

N/A

Storage conditions

- 70°C

Technical datasheet

Contact us to receive the datasheet

Shipping condition

dry ice

Warnings

For Research Use only.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus,Rattus norvegicus