Mouse anti-IgE (IgG), monoclonal
890 EUR
1 mg lyophilized
12 400 010
Monoclonal Antibody
Monoclonal antibody to human IgE. Immunoglobulin isotype: IgG1. The monoclonal antibody was raised against recombinant human IgE. The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography.
mouse
Autoimmune disease
lyophilized
Monoclonal
recombinant human IgE
Human
See the data sheet or contact us
PBS, with 0,1% Azide
>95% pure. Purity is tested by electrophoresis.
• ELISA • Immunoblotting
Mab to human IgE. Immunoglobulin isotype: IgG1. The monoclonal antibody was raised against recombinant human IgE. The immune lymphocytes from BALB/c mice were extracted from lymph nodes and fused with myeloma sp2/0 cells according to a standard protocol. The IgG1 fraction was purified by Protein G affinity chromatography.
no crossreactivty to IgA, IgM, IgG
recombinant and human IgE
Protein G purified
1. Strohner P: Patent: EP 1957980B1 Immunoassay for the simultaneous immunochemical determination of an analyte (antigen) and a treatment antibody targeting the analyte in samples (Recovery immunoassay) 2. Korn S, Haasler I, Jung M, Strohner P, Staatz A, Schäfer A, Becher G, Taube C and Buhl R Free serum IgE in patients with severe allergic asthma treated with Omalizumab Am. J. Respir. Crit. Care Med., May 2010; 181: A6655 3. Strohner P, Sarrach D, Reich JG, Becher G, Staatz A, Schäfer A, Steiß J-O, Häupl T: The Recovery-ELISA – a novel Assay Technique for the Therapy Control of Therapeutic Antibodies Am J Respir Crit Care Med 181;2010:A5674 4. Strohner P, Staatz A, Schäfer A, Becher G, Sarrach D, Reich JG, Steiß J-O, Häupl T: Monitoring of Monoclonal Antibody Therapy with a new Recovery ELISA Assay Technique (R-ELISA®) ERS Barcelona 2010, Abstract-No: 3399 5. Steiß JO, Schmidt A, Lindemann H, Rudloff S, Staatz A, Strohner P, Becher G, Nährlich L, Zimmer KP: Therapiemonitoring von Omalizumab im Kindes- und Jugendalter. Allergologie, Jahrgang 33, Nr. 10/2010, S. 421–428
N/A
-20°C long term; +4°C short term
Contact us to receive the datasheet
-80°C +37°C
For Research Use only.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
IgE,IgG
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus
