Anti-Rabbit IgG, H&L Chain Specific-FITC Polyclonal antibody

Basic information

  • Name

    Anti-Rabbit IgG, H&L Chain Specific-FITC Polyclonal antibody

  • Price

    218 EUR

  • Size

    1mg

  • Catalog number

    81301

More detailed information

Clonality

Polyclonal antibody

Long name

Polyclonal antibody against Rabbit IgG, H&L Chain Specific-FITC

Aplication

You will receive the antybody in PBS(7,4 pH), for future delutions PBS or mediun similar to the one used for the assay are suitable.

Antigen:

Rabbit IgG, H&L Chain Specific-FITC

Storage

Could be stored for at least 12 month in frozen condition at -70C.It is recomendable to prepare aliquotes in order to avoid repetable freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.

Product description

Anti-Rabbit IgG, H&L Chain Specific-FITC Polyclonal antibody can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by QED Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This QED Biosciences Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. QED Biosciences FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Additional isotype

Heavy & Light chains,IgG

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by QED Biosciences. Rabbit antibodies are very stable and can be stored for several days at room temperature. QED Biosciences adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus

French translation

anticorps