Rabbit Monoclonal IgG Anti-CD117 (SCFR, c-kit Onkoprotein) [YR145]

Basic information

  • Name

    Rabbit Monoclonal IgG Anti-CD117 (SCFR, c-kit Onkoprotein) [YR145]

  • Price

    436 EUR

  • Size

    0,5 ml

  • Catalog number

    CD11702-0.5

More detailed information

Availability

Please contact us to check the availability

Immunogen

A synthetic peptide from C-terminus of human CD117 / c-kit protein

Specificity

Human CD117, c-kit Onkoprotein, SCFR (Stem Cell Factor Receptor) 145 kDa in WB

Species reactivity

Human, Dog

Product presentation

Cell culture supernatant containing sodium azide 0.09%.

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

YR145

Product is supplied as

liquid concentrate

Recommended concentration for use

1:50-1:250

Protein concentration

S/N

Positive control

Skin, bone marrow, GIST

Pretreatment

We recommend high temperature unmasking with Unmasking Fluid E (Tris-buffered EDTA solution pH 8.2, Art. No. DE006), which is advantageous to citrate buffer pre-treatment.

Tested applications

IHC(C, P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

P1Go to UniProt/SwissProt721 - KIT_HUMAN

Litterature

1. Natali P.G., Nicotra M.R., Winkler A.B., et al. (1992) Progression of human cutaneous melanoma is associated with loss of expression of c-kit proto-oncogene receptor. Int. J. Cancer 52; 197-201. 2. Matsuda R., Takahashi T., Nakamura S., et al. (1993) Expression of the c-kit protein in human solid tumors and in corresponding fetal and adult normal tissues. Am. J. Pathol. 142(1); 339-346. 3. Izquierdo M.A., Van der Valk P., Van Ark-Otte J. et al. (1995) Differential expression of the c-kit proto-oncogene in germ cell tumors. J. Pathol. 177; 253-258. 4. Miettinen M., and Lasota J. (2003) Gastrointestinal stromal tumors (GISTs): definition, occurence, pathology, differential diagnosis and molecular genetics. Pol J. Pathol. 54(1); 3-24.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus