Rabbit Monoclonal IgG Anti-ERG (TMPRSS2-ERG) [EPR3864]

Basic information

  • Name

    Rabbit Monoclonal IgG Anti-ERG (TMPRSS2-ERG) [EPR3864]

  • Price

    382 EUR

  • Size

    5 ml

  • Catalog number

    ERG001-G

More detailed information

Availability

Please contact us to check the availability

Immunogen

C-terminal synthetic peptide of human ERG

Specificity

Human and murine ERG, detects also Fli-1

Species reactivity

Human, mouse, rat

Product presentation

Protein A purified antibody in PBS, BSA, sodium azide (0,09%)

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

EPR3864

Product is supplied as

ready to use

Recommended concentration for use

RTU

Positive control

ERG-positive prostate carcinoma

Pretreatment

Pre-treatment of dewaxed slices with heat induced epitope retrieval method is recommended. Use TRIS/Borate/EDTA buffer, Citrate Buffer (code no. DE000) or Unmasking Solution G (code no. DE007) .

Tested applications

IHC(C,P), ICC, WB

Recommended secondary reagent

Anti-rabbit IgG:Biotin conjugate (code no. ZU101) in conjunction with Streptavidin-HRPO (code no. ZU054) or VECTOR ImmPress rabbit IgG Kit (code no. MP-7401).

Storage method

Store at 2-8°C

UniProt number

B2Y833 (B2Y833_HUMAN)

Litterature

1. Park K, Tomlins SA, Mudaliar KM, Chiu YL, Esgueva R, Mehra R, Suleman K, Varambally S, Brenner JC, MacDonald T, Srivastava A, Tewari AK, Sathyanarayana U, Nagy D, Pestano G, Kunju LP, Demichelis F, Chinnaiyan AM, Rubin MA. Antibody-based detection of ERG rearrangement-positive prostate cancer. Neoplasia. 2010 Jul;12(7):590-598. 2. Chaux,A., Albadine,R., Toubaji,A., Hicks,J., Meeker,A., Platz,E.A et al. (2011) Immunohistochemistry for ERG expression as a surrogate for TMPRSS2-ERG fusion detection in prostatic adenocarcinomas, Am. J. Surg. Pathol. 35 (7), 1014-1020 PUBMED 21677539 3. Yaskiv,O., Zhang,X., Simmerman,K., Daly,T., He,H., Falzarano,S., Chen,L., Magi-Galluzzi,C. and Zhou,M. (2011) The utility of ERG/P63 double immunohistochemical staining in the diagnosis of limited cancer in prostate needle biopsies, Am. J. Surg. Pathol. 35 (7), 1062-1068 PUBMED 21623182 4. Miettinen,M., Wang,Z.F., Paetau,A., Tan,S.H., Dobi,A., Srivastava,S. and Sesterhenn,I. (2011) ERG transcription factor as an immunohistochemical marker for vascular endothelial tumors and prostatic carcinoma, Am. J. Surg. Pathol. 35 (3), 432-441 PUBMED 21317715 5. Queisser A., Hagedorn S.A., Braun M., Vogel W., Duensing S., Perner S. (2015) Comparison of different prostatic markers in lymph node and distant metastases of prostate cancer. ModernPathology 28, 138–145.

Tips

Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Latin name

Oryctolagus cuniculus