Rabbit Polyclonal IgG Anti-Fibronectin, mouse

Basic information

  • Name

    Rabbit Polyclonal IgG Anti-Fibronectin, mouse

  • Price

    320 EUR

  • Size

    0.1 ml

  • Catalog number

    FI24951-0.1

More detailed information

Availability

Please contact us to check the availability

Immunogen

Purified fibronectin from murine plasma

Specificity

Murine fibronectin 100%, murine collagen type I, III and IV

Species reactivity

Mouse

Product presentation

Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

polyclonal

Product is supplied as

purified, lyophilized

Recommended concentration for use

IFA ≥ 1:80, IHC(P) ≥ 1:1000, ELISA ≥ 1:200 (OD ≥ 500)

Protein concentration

app. 1 mg/ml

Positive control

Mouse skin

Pretreatment

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Tested applications

IFA, IHC(P), ELISA, RIA

Recommended secondary reagent

Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).

Storage method

Store at -20°C

UniProt number

P11276 (FINC_MOUSE)

Litterature

1. Andujar MB, Magloire H, Hartmann DJ, Ville G, Grimaud JA (1985) Early mouse molar root development: cellular changes and distribution of fibronectin, laminin and type-IV collagen. Differentiation. 30(2):111-22. 2.Savino W, Itoh T, Imhof BA, Dardenne M (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus. 8(4):245-56. 3. Villa-Verde DM1, Lagrota-Candido JM, Vannier-Santos MA, Chammas R, Brentani RR, Savino W (1994) Extracellular matrix components of the mouse thymus microenvironment. IV. Modulation of thymic nurse cells by extracellular matrix ligands and receptors. Eur J Immunol. 24(3):659-64. 4. Pankov R and Yamada KM (2002) Fibronectin at a glance. J Cell Sci. Oct 15;115(Pt 20):3861-3 5. Labat-Robert J (2002) Fibronectin in malignancy. Semin Cancer Biol. Jun;12(3):187-95.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus,Oryctolagus cuniculus

Group

Polyclonals and antibodies