Rabbit Polyclonal IgG Anti-Mammaglobin, polycl.

Basic information

  • Name

    Rabbit Polyclonal IgG Anti-Mammaglobin, polycl.

  • Price

    540 EUR

  • Size

    0.5 ml

  • Catalog number

    MAM003-0.5

More detailed information

Availability

Please contact us to check the availability

Immunogen

C-terminal 16 aa peptide sequence of human mammaglobin coupled to KLH as a carrier protein

Specificity

10 KDa Mammaglobin from human breast tissue, no cross-reactivity with mammaglobin B, lipophilin and uteroglobin

Species reactivity

Human, others not tested

Product presentation

Affinity purified antibody in stabilized phosphate buffer, containing 0.09 % sodium azide**. The volume is sufficient for app. 250 immunohistochemical tests (100 µl working solution per test). For dilution we recommend our antibody diluent Art. No. PU002.

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

polyclonal

Product is supplied as

liquid concentrate

Recommended concentration for use

1:50

Protein concentration

50 µg/ml

Positive control

Mamma carcinoma (normal mamma shows up lower expression of the protein), skin sweat glands

Pretreatment

Formalin-fixed tissue may be pre-treated for 30 min at 100°C with Unmasking Solution C (Citrate buffer Art. No. DE000), E (buffered EDTA Art. No. DE006) or G (Glycol based solution Art. No. DE007) preferably in a pressure cooker.

Tested applications

IHC, ELISA , WB

Recommended secondary reagent

Gentaur Universal Staining Kits Art.-No. DA005 (AE005) or VECTASTAIN Elite ABC Rabbit IgG (Art.-No. PK-6101) or VECTOR ImmPRESS™ Rabbit IgG Kit (Art. No. MP-7401)

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Watson MA, Dintzis S, Darrow CM, Voss LE, DiPersio J, Jensen R, Fleming TP. Mammaglobin expression in primary, metastatic, and occult breast cancer. Cancer Res. 1999 Jul 1;59(13):3028-31. 2. Fanger GR, Houghton RL, Retter MW, Hendrickson RC, Babcook J, Dillon DC,Durham MD, Reynolds LD, Johnson JC, Carter D, Fleming TP, Roche PC, Persing DH, Reed SG. Detection of mammaglobin in the sera of patients with breast cancer. Tumour Biol. 2002 Jul-Aug;23(4):212-21. 3. Han JH, Kang Y, Shin HC, Kim HS, Kang YM, Kim YB, Oh SY. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med. 2003 Oct;127(10):1330-4. 4. Gillanders WE, Mikhitarian K, Hebert R, et al. Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg. 2004 Jun;239(6):828-37; discussion 837-40. 5. Sjödin A., et al. Mammaglobin in normal human sweat glands and human sweat gland tumors. Journal of Investigative Dermatology (2003) 121(2): 428-429.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus