Rabbit Polyclonal IgG Anti-NF kappa B / p65

Basic information

  • Name

    Rabbit Polyclonal IgG Anti-NF kappa B / p65

  • Price

    0 EUR

  • Size

    100 µg

  • Catalog number

    NFK001

More detailed information

Availability

Please contact us to check the availability

Immunogen

Synthetic peptide from internal domain of NFkB p65

Specificity

NFkB (65kDa)

Species reactivity

Human, mouse, rat (other vertebrates expected, but not tested)

Product presentation

Affinity purified IgG fraction from rabbit serum in PBS containing 0.2% BSA and 0.09% NaN3

Product category

Primary Antibody

Antibody's raised in

Rabbit

Antibody's isotype

IgG

Antibody's clone

polyclonal

Product is supplied as

liquid concentrate

Recommended concentration for use

IHC (2 µg/ml), WB (2-5 µg/ml)

Protein concentration

200 µg/ml

Positive control

Prostate, placenta

Pretreatment

Unmasking with citrate buffer pH 6 10-20 min at 100°C

Tested applications

IHC(P), WB

Recommended secondary reagent

Biotinylated anti-Rabbit IgG (Art. No. ZU101) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051) and a suitable substrate.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Additional isotype

IgG

Group

Polyclonals and antibodies

Latin name

Oryctolagus cuniculus